6/2/2023 0 Comments Free to print rat target![]() LINC00578 positively regulated cell proliferation and invasion in vitro and tumorigenesis in vivo in pancreatic cancer. An immunohistochemical assay was used to confirm the correlation between LINC00578 and SLC7A11 in the clinic. Coimmunoprecipitation assays were used to investigate the association of LINC00578 with SLC7A11 in ubiquitination and to confirm the interaction between ubiquitin-conjugating enzyme E2 K (UBE2K) and SLC7A11. Pull-down and RNA immunoprecipitation assays were carried out to determine and validate the binding protein of LINC00578. Label-free proteomic analysis was performed to select LINC00578-related differentially expressed proteins. A series of loss- and gain-of-function experiments in vitro and in vivo were performed to explore the oncogenic role of LINC00578 in pancreatic cancer development and progression. In this study, we discovered that LINC00578 acted as a regulator of ferroptosis in pancreatic cancer. ![]() lncRNAs play crucial roles in regulating the biological behaviors of tumor cells. Pancreatic cancer is a highly aggressive malignancy worldwide with rapid development and an exceedingly poor prognosis.
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